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甘蔗与斑茅BC1分子鉴定、抗黑穗病和花叶病初步评价
吴小斌, 王勤南, 凌秋平, 杨湛端, 陈勇生, 黄俊坚, 吴嘉云, 李奇伟
广东省生物工程研究所(广州甘蔗糖业研究所), 广东省甘蔗改良与生物炼制重点实验室, 广州 510316
摘要:
为了解甘蔗(Saccharum)与斑茅(Erianthus arundinaceus)杂交后代作为抗病亲本的利用价值,通过特异引物PCR鉴定出78份甘蔗与斑茅杂交BC1真实杂种;通过人工接种花叶病毒和黑穗病菌,初步评价了甘蔗和斑茅杂交BC1的抗病表现。结果表明,甘蔗和斑茅杂交BC1的抗花叶病具有普遍性,而黑穗病抗性则出现分离。初步筛选出BC1无性系YCE01-48、YCE01-71、YCE01-105、YCE01-125、YCE02-184和YCE01-118可同时抗花叶病和黑穗病,有望成为甘蔗杂交利用的高抗病源亲本。
关键词:  甘蔗  斑茅  分子鉴定  抗病
DOI:10.11926/jtsb.3926
分类号:
基金项目:国家自然科学基金项目(31571730,31701488,31401440);广东省科技计划项目(2014A030304027,2015A030302030,2017A030303048);广东省自然科学基金项目(2015A030313696,2015A030310286);国家糖料产业技术体系项目(CARS-170107);广东省科学院专项资金(2017GDASCX-0105)资助
Molecular Identification and Resistance Evaluation to Smut and Mosaic Disease with BC1 of Saccharum×Erianthus arundinaceus
WU Xiao-bin, WANG Qin-nan, LING Qiu-ping, YANG Zhan-duan, CHEN Yong-sheng, HUANG Jun-jian, WU Jia-yun, LI Qi-wei
Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangdong Provincial Bioengineering Institute(Guangzhou Sugarcane Industry Research Institute), Guangzhou 510316, China
Abstract:
In order to understand the utilization value of hybrids between Saccharum and Erianthus arundinaceus as resistant parents, the resistance to smut and SCMV of some progenies BC1 from Saccharum×E. arundinaceus were evaluated by artificial inoculation of pathogens. The results showed that most of BC1 had the resistance to SCMV, and the resistance to smut was segregative among progenies. The BC1 clones YCE01-48, YCE01-71, YCE01-105, YCE01-125, YCE02-184 and YCE01-118 showed co-resistance to smut and SCMV. So, they could be used as parents with high disease resistance for sugarcane breeding.
Key words:  Saccharum  Erianthus arundinaceus  Molecular identification  Disease resistance

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