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巴西橡胶树 HbERFB4-1 基因的克隆和表达分析
朱家红1,2, 邱琼1,2, 畅文军3, 张治礼4,5
1. 海南大学农学院;2. 中国热带农业科学院热带生物技术研究所, 农业部热带作物生物技术重点开放实验室;3.中国热带农业科学院热带生物技术研究所, 农业部热带作物生物技术重点开放实验室;4.1. 中国热带农业科学院热带生物技术研究所, 农业部热带作物生物技术重点开放实验室;5.2. 海南省农业科学院
摘要:
根据EST序列信息,利用RACE技术分离了巴西橡胶树(Hevea brasiliensis)的1个AP2/ERF类基因,命名为HbERFB4-1。该基因cDNA全长732 bp,含有完整的开放阅读框架,编码147个氨基酸,不含内含子。推导的HbERFB4-1氨基酸序列与杨树(Populus trichocarpa)、蓖麻(Ricinus communis)、拟南芥(Arabidopsis thaliana)和大豆(Glycine max)中相应蛋白氨基酸序列的一致性分别为78%、71%、62%和55%,具有典型的AP2类蛋白结构特征。聚类分析表明,HbERFB4-1属于ERF家族B4亚族。半定量RT-PCR分析结果表明,该基因在胶乳中的表达量相对较低,但乙烯利刺激后其在胶乳中的表达量迅速增加,推测该基因可能参与了胶乳中乙烯信号的转导。
关键词:  巴西橡胶树  AP2/ERF类基因  乙烯利  基因表达
DOI:10.3969/j.issn.1005-3395.2012.02.006
分类号:
基金项目:国家自然科学基金项目(31000313);海南省自然科学基金项目(309052);中央级公益性科研院所业务费专项资金资助项目(ITBB110201)资助
Cloning and Expression Analysis of HbERFB4-1 Gene in Hevea brasiliensi
ZHU Jia-hong1,2, QIU Qiong1,2, CHANG Wen-jun3, ZHANG Zhi-li4,5
1. College of Agronomy, Hainan University, China;2. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, China;3.Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, China;4.1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, China;5.2. Hainan Academy of Agricultural Sciences, China
Abstract:
Based on EST sequences from an ethephon-induced latex SSH cDNA library of Hevea brasiliensis, a full-length cDNA encoding AP2/ERF type gene named as HbERFB4-1, was cloned from H. brasiliensis by RACE-PCR. The HbERFB4-1 cDNA has full length of 732 bp with an open reading frame (ORF) of 444 bp, and encodes 147 amino acid residues. No introns were detected in HbERFB4-1gene. The deduced HbERFB4-1 contains an AP2 domain and three NISs, which shows high identity to AP2/ERF in Populus trichocarpa,Ricinus communis,Arabidopsis thaliana and Glycine max, counting for 78%、71%、62% and 55%, respectively. Phyogenetic analysis showed that HbERFB4-1 belonged to the B4 subfamily of ERF family. Semi-quantitative RT-PCR analysis indicated that the transcription of HbERFB4-1 in latex was low, but it was rapidly induced by ethephon, suggesting that HbERFB4-1 is involved in the ethylene signal pathway in latex of H. brasiliensis.
Key words:  Hevea brasiliensis  AP2/ERF type gene  Ethephon  Gene expression

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