Home | Brief Introduction | Information for Authors | Editorial Board | Subscription | Contact us | 中文版
 
   
  News
Visiting Times
4003021
Visiting Times of Today
57
Now Online
0
 
引用本文:
【打印本页】   【下载PDF全文】   View/Add Comment  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 3666次   下载 1876 本文二维码信息
码上扫一扫!
分享到: 微信 更多
葎草雄性连锁的ISSR标记克隆及SCAR标记的建立
高武军, 沙涛, 姬艳克, 邓传良, 卢龙斗
河南师范大学生命科学学院
摘要:
以雌雄异株植物葎草(Humulus scandens L.)为材料,通过优化扩增体系中的退火温度、Mg2+浓度及模板浓度等主要影响因素,利用简单重复序列间扩增标记对其雌雄株性别的基因组差异进行研究。结果表明,62条ISSR引物中有40条引物能扩增出稳定的条带,共产生302条带。引物I64扩增出1条雄性连锁标记,经克隆测序,该片段长度为553 bp,AT含量为64.3%,根据测序结果设计特异引物,将该标记转化为稳定性更好的SCAR标记。
关键词:  葎草  ISSR  雄性连锁  SCAR
DOI:10.3969/j.issn.1005-3395.2010.3.2425
分类号:
基金项目:国家自然科学基金项目(30970211); 河南省自然科学基金项目(2006180014)
Clone and Development of ISSR and SCAR Markers Linked to Male Humulus scandens L.
GAO Wu-jun, SHA Tao, JI Yan-ke, DENG Chuan-liang, LU Long-dou
College of Life Science,Henan Normal University
Abstract:
The sex differences in Humulus scandens were studied by using inter-simple sequence repeat (ISSR) technique. 40 primes from total 62 primers could produce stable bands, and amplified 302 bands. A male-linked fragment with about 553 bp was amplified by I64 primer which was abundant in AT (64.3%). Then the male-linked marker was converted into SCAR marker.
Key words:  Humulus scandens L.  ISSR  Male-linked  SCAR

Copyright 2007 South China Botanical Garden
Addresss: Xingke Road 723,Tianhe District,Guangzhou,China Post code:510650
Telephone:+86 (0)20-3725 2514  Fax: +86 (0)20-3725 2642 E-mail: jtsb@scib.ac.cn
Designed by Beijing E-Tiller Co.,Ltd