首页 | 本刊简介 | 编委会 | 投稿征稿 | 期刊订阅 | 下载中心 | English
 
   
  论文快速检索

请输入关键词

 

 

   
  信息公告
海外撷英栏目开通,欢迎投稿

热带亚热带植物学报网络开通

访问总次数
14820608
今日访问
4573
当前在线
0人
 
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 24次   下载 6 本文二维码信息
码上扫一扫!
分享到: 微信 更多
巨桉BRI1基因的克隆和表达分析
李晓丹, 胡冰, 裘珍飞, 范春节, 闫慧芳, 曾炳山
中国林业科学研究院热带林业研究所, 广州 510520
摘要:  为了解BRI1基因在巨桉中的功能,采用PCR技术克隆了EgrBRI1基因,分析了EgrBRI1的生物信息学和亚细胞定位,并对EgrBRI1基因响应激素和胁迫的差异表达进行了分析。结果表明,EgrBRI1基因全长3 893 bp,编码1 197个氨基酸。EgrBRI1蛋白稳定,空间结构复杂,存在3个motifs,主要定位于细胞膜。茉莉酸甲酯和油菜素内酯(BR)处理后,EgrBRI1基因在叶片中的表达上升,而水杨酸处理则没有明显的变化。盐胁迫和冷胁迫下,EgrBRI1基因表达表现为先下降后上升的趋势。因此,EgrBRI1基因能快速对外施激素做出响应,并在巨桉抗逆方面发挥重要作用,这可能是通过对BR信号的响应来实现的。
关键词:  巨桉  油菜素内酯  EgrBRI1基因  基因表达
DOI:10.11926/jtsb.4044
分类号:
基金项目:国家自然科学基金项目(31400554)资助
Gene Cloning and Expression Analysis of BRI1 in Eucalyptus grandis
LI Xiao-dan, HU Bing, QIU Zhen-fei, FAN Chun-jie, YAN Hui-fang, ZENG Bing-shan
Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guagndong, Guangzhou 510520, China
Abstract:  In order to understand the function of BRI1 gene in Eucalyptus grandis, EgrBRI1 gene was cloned, and the bioinformatics and subcellular localization of EgrBRI1 were analysis. Meanwhile, the expression of EgrBRI1 gene were analyzed after treated with hormones and stresses by qRT-RCR. The results showed that the length of EgrBRI1 gene was 3 893 bp, encoding 1 197 amino acids. EgrBRI1 protein was relatively stable with complex spatial structure, which contains three motifs. EgrBRI1 protein was mainly located in cell membrane. When treated with methyl jasmonate and brassinolide, the expression of EgrBRI1 gene in leaves showed an up-regulated trend with the time, while there was no significant change by treated with salicylic acid. Under salt and cold stresses, the expression of EgrBRI1 gene was down-regulated at first and then increased with the time. These indicated that EgrBRI1 gene could respond quickly to applied hormone and play an important role in stress resistance of E. grandis, which might be achieved by responding to BR signal.
Key words:  Eucalyptus grandis  Brassinosteroid  EgrBRI1 gene  Gene expression

Copyright 2007 版权所有 中国科学院华南植物园《热带亚热带植物学报》编辑部
地址: 广州市天河区兴科路723号 中国科学院华南植物园 《热带亚热带植物学报》编辑部 邮编:510650 电话: 86 (0)20-3725 2514  传真:86 (0)20-3725 2642 E-mail: jtsb@scib.ac.cn
本系统由北京勤云科技发展有限公司设计
京ICP备09084417号-2