首页 | 本刊简介 | 编委会 | 投稿征稿 | 期刊订阅 | 下载中心 | English
 
   
  论文快速检索

请输入关键词

 

 

   
  信息公告
海外撷英栏目开通,欢迎投稿

热带亚热带植物学报网络开通

访问总次数
14820601
今日访问
4569
当前在线
0人
 
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 24次   下载 8 本文二维码信息
码上扫一扫!
分享到: 微信 更多
不同成熟度树葡萄叶片中类黄酮合成转录组基因分析
张少平1,2, 郑开斌1,2, 洪佳敏1, 林宝妹1, 张帅1, 邱珊莲1
1.福建省农业科学院亚热带农业研究所, 福建 漳州 363005;2.福建省农业科学院作物研究所, 福州 350013
摘要:  为了解树葡萄(Myrciaria cauliflora)类黄酮合成相关酶差异表达基因信息,对其幼叶和成熟叶进行全转录组测序并比较分析。结果表明,从树葡萄幼叶和成熟叶中获得59 321条单基因簇(Unigenes),在8大数据库共注释到32 912条Unigenes信息,其中类黄酮合成代谢相关酶基因77个,在成熟叶片中显著下调表达的基因6个,包括2个CHI、1个CHS、1个F3H、1个2-羟基异黄酮脱水酶基因和1个ANS。5个差异表达基因经qRT-PCR验证的结果与转录组测序结果相符合。因此,树葡萄叶片中含有大量不同种类黄酮合成代谢相关酶家族基因,成熟叶片中类黄酮含量显著减少是由于少量相关基因显著下调。
关键词:  树葡萄  叶片  类黄酮  转录组基因
DOI:10.11926/jtsb.4040
分类号:
基金项目:福建省农业科学院创新团队项目(STIT2017-2-11);福建省农业科学院青年创新团队项目(STIT2017-3-14);福建省公益类科研院所专项(2017R1024-1)资助
Transcriptome Analysis of Flavonoid Synthesis Related Genes in Different Maturity Leaves of Myrciaria cauliflora
ZHANG Shao-ping1,2, ZHENG Kai-bin1,2, HONG Jia-min1, LIN Bao-mei1, ZHANG Shuai1, QIU Shan-lian1
1.Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, Fujian, China;2.Crop Sciences Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
Abstract:  The flavonoid content in immature leaves was significantly higher than that in mature leaves of Myrciaria cauliflora. In order to understand the information of differentially expressed genes related flavonoid synthesis in M. cauliflora, the transcriptome of 2 kinds of leaves were sequenced by Illumina HiSeq method. The results showed that there were 59 321 Unigenes obtained from young and mature leaves, among which 32 912 unigenes were annotated by 8 databases. There were 77 Unigenes related flavonoid anabolism, and 6 genes were down-regulated significantly in mature leaves, including two CHIs, one CHS, one F3H, one 2-hydroxyiso-flavanone dehydratase gene and one ANS. The RT-PCR verified results of five differentially expressed genes were agreed with the transcriptome sequencing. So, it was suggested that there were a large number of family genes related flavonoid anabolism in the leaves of M. cauliflora, but the decrease of flavonoid content in mature leaves was due to several down-regulated genes.
Key words:  Myrciaria cauliflora  Leaf  Flavonoid  Transcriptome gene

Copyright 2007 版权所有 中国科学院华南植物园《热带亚热带植物学报》编辑部
地址: 广州市天河区兴科路723号 中国科学院华南植物园 《热带亚热带植物学报》编辑部 邮编:510650 电话: 86 (0)20-3725 2514  传真:86 (0)20-3725 2642 E-mail: jtsb@scib.ac.cn
本系统由北京勤云科技发展有限公司设计
京ICP备09084417号-2