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黄藤染色体核型及基因组大小分析
史晶晶1,2, 徐瑞晶1,3, 徐浩1,2, 马霜1,2, 贾秋蕊4, 高志民1,2
1.国家林业局竹藤科学与技术重点开放实验室, 北京 100102;2.国际竹藤中心竹藤资源基因科学研究所, 北京 100102;3.国际竹藤中心热带森林植物研究所, 海南 三亚 572000;4.河北省唐山市迁西县林业局, 河北 唐山 064300
摘要:  为探究黄藤(Daemonorops jenkinsiana)染色体核型和基因组的大小,采用体细胞染色体常规制片法与显微摄影技术相结合的方法,对黄藤染色体进行了核型分析,同时以番茄(Lycopersicon esculentum)为内标,应用流式细胞术对黄藤叶片基因组大小、DNA含量和DNA倍性进行了测定。结果表明,黄藤茎尖是理想的染色体制片材料;黄藤的染色体数为2n=24,核型公式为K(2n)=1M+17m+5sm+1st,核型类型为2C;核型不对称系数61.20%;黄藤的DNA含量为1.57 pg,基因组大小为1 539.53 Mb,黄藤的DNA倍性为二倍体(2n)。这是首次报道黄藤的核型和基因组大小,为深入开展黄藤属及其近缘属植物的核型和基因组比较分析提供了参考依据。
关键词:  黄藤  染色体  核型  基因组
DOI:10.11926/jtsb.3982
分类号:
基金项目:“十二五”农村领域国家科技计划项目(2015BAD04B0101)资助
Karyotype and Genome Size Analyses of Daemonorops jenkinsiana
SHI Jing-jing1,2, XU Rui-jing1,3, XU Hao1,2, MA Shuang1,2, JIA Qiu-rui4, GAO Zhi-min1,2
1.State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Beijing 100102, China;2.Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan(ICBR), Beijing 100102, China;3.Institute of Tropical Forest Plant, ICBR, Sanya 572000, Hainan, China;4.Qianxi County Forestry Bureau, Tangshan 064300, Hebei, China
Abstract:  In order to explore the karyotype and genome size of Daemonorops jenkinsiana, the root and stem tips from its seedlings were used as experimental materials. The karyotype of D. jenkinsiana, including chromosome number and morphology were analyzed by combining the conventional method of somatic cell chromosome preparation with microphotography. Meanwhile, the DNA content, genome size and ploidy of leaves were determined by flow cytometry using Lycopersicon esculentum as an internal reference. The results showed that the stem tip of D. jenkinsiana was an ideal material for chromosome preparation, and the chromosome number was 2n=24, along with the formula of karyotype was K(2n)=1M+17m+5sm+1st, and the karyotype was "2C". The karyotype asymmetry index of D. jenkinsiana was 61.20%. The DNA content of D. jenkinsiana was about 1.57 pg with the genome size of 1 535.53 Mb, and the DNA ploidy was diploid (2n). This was reported the karyotype and genome size of D. jenkinsiana for the first time, which provided a reference for studying the karyotype and genome of other species in Daemonorops and related genus.
Key words:  Daemonorops jenkinsiana  Chromosome  Karyotype  Genome

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