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冰菜盐胁迫下的转录组分析
练冬梅, 赖正锋, 姚运法, 林碧珍, 洪建基
福建省农业科学院亚热带农业研究所, 福建漳州 363005
摘要:  为了解冰菜(Mesembryanthemum crystallinum)叶片抗盐相关基因组学,利用Illumina Hi-seq TM2500高通量测序技术研究冰菜叶片在400 mmol L-1 NaCl胁迫下转录组基因的差异表达。结果表明,从400 mmol L-1 NaCl胁迫和对照的冰菜叶片中共获得13.01 Gb Clean data,Q30碱基均大于90.08%。共获得123个差异表达基因(DEGs),包括73个上调基因,50个下调基因,其中功能注释的基因有96个。根据Unigene库序列进行GO、COG和KEGG注释,筛选出8个与抗盐性相关差异表达基因,植物激素代谢相关基因,脱落酸8'-羟基化酶、吲哚-3-乙酰酸酰胺合成酶和茉莉酮酸酯ZIM结构域蛋白基因均下调表达,生长素响应蛋白、细胞分裂素合酶基因则上调表达,糖代谢相关基因棉子糖合成酶基因上调表达,质膜H+-ATPase基因上调表达,脱水蛋白基因下调表达。这为冰菜耐盐基因组学和分子生物学的研究奠定基础。
关键词:  冰菜  盐胁迫  叶片  转录组  差异表达基因
DOI:10.11926/jtsb.3972
分类号:
基金项目:福建省公益类科研院所专项(2018R1101025-2)资助
Transcriptome Analysis of Mesembryanthemum crystallinum under Salt Stress
LIAN Dong-mei, LAI Zheng-feng, YAO Yun-fa, LIN Bi-zhen, HONG Jian-ji
Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou 363005, Fujian, China
Abstract:  In order to understand the salt related genomics in leaves of Mesembryanthemum crystallinum, the expression of transcriptome genes was studied under 400 mmol L-1 NaCl stress by using Illumina Hiseq TM2500 high-throughput sequencing technology. The results showed that there were 13.01 Gb Clean Data from the leaves treated with 400 mmol L-1 NaCl and untreated, the Q30 base was more than 90.08%. Among them, there were 123 differential expression genes (DEGs), including 73 up-regulated genes and 50 down-regulated genes, in which 96 genes were annotated. The eight salt-resistance DEGs of were screened out based on GO, COG and KEGG annotation. The plant hormone metabolism-related genes, including abscisic acid 8'-hydroxylase gene, indole-3-acetamide synthase gene, jasmonate ZIM structure domain protein gene were down-regulated, auxin responsive protein and cytokinin synthase genes were up-regulated. The sugar metabolism-related raffinose synthase genes was up-regulated, and plasma membrane H+-ATPase and dehydrated protein genes were up and down-regulated, respectively. These would provide a foundation for studing on salt tolerance genomics and molecular biology of M. crystallinum.
Key words:  Mesembryanthemum crystallinum  Salt stress  Leaf  Transcriptome  DEGs

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