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不同水分条件下杜鹃花转录因子的转录组分析
王华1, 张石虎1, 龚雪梅2, 王瑞生1, 邓新义1
1.安徽农业大学园艺学院, 合肥 230036;2.阜阳职业技术学院生化工程系, 安徽 阜阳 236031
摘要:  为了解杜鹃花(Rhododendron pulchurum)转录因子在干旱胁迫下的表达模式,利用高通量测序技术,对不同水分条件下“白凤4号”叶的转录因子表达谱进行分析。结果表明,在不同水分处理间有34~161个差异表达的转录因子,杜鹃花响应不同水分的转录调控主要是通过ERFbHLHMYB基因的表达协同完成的;干旱胁迫时,特异调动了NAC的差异表达和WRKYbZIPPLATZ的上调表达,干旱后复水时特异调动了GATA表达来调控。RT-qPCR验证结果表明,基因表达趋势与测序结果一致,证明了测序结果的有效性。这为明晰杜鹃花抗旱的分子机理及分子育种奠定了理论基础。
关键词:  杜鹃花  转录因子  转录组  干旱胁迫
DOI:10.11926/jtsb.3910
分类号:
基金项目:安徽省高校自然科学研究项目(KJ2016A237,KJ2016A558)资助
Transcriptome Analysis of Transcription Factors of Rhododendron pulchurum ‘Baifeng4’ under Different Water Conditions
WANG Hua1, ZHANG Shi-hu1, GONG Xue-mei2, WANG Rui-sheng1, DENG Xin-yi1
1.College of Horticulture, Anhui Agricultural University, Hefei 230036, China;2.Department of Biochemical Engineering, Fuyang Institute of Technology, Fuyang 236031, Anhui, China
Abstract:  In order to reveal the expression pattern of transcriptome factors (TFs) of Rhododendron pulchurum under drought stress, the expression profiles of transcription factors in ‘Baifeng 4’ under different water conditions were analysed by using the high-throughput sequencing technology. The results showed that there were 34-161 differential expression TFs among different water treatments. The transcriptional regulation in Rhododendron response to drought was synergistic effects of the expressions of ERF, bHLH and MYB genes. The special expression of NAC and up expression of WRKY, bZIP, PLATZ were found under drought stress, and the special expression of GATA in rehydration. The trend of gene expression was consistent with sequencing results by RT-qPCR, showing the validity of sequencing results. Therefore, these would be lay a theoretical foundation for understanding the molecular mechanism of drought resistance and molecular breeding of Rhododendron.
Key words:  Rhododendron  Transcription factor  Transcriptome  Drought stress

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