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巴西橡胶树栽培种质基因组C值测定和变异分析
柳觐, 牛迎凤, 吴裕, 毛常丽, 张凤良, 刘紫艳, 郑诚, 龙青姨, 李国华
云南省热带作物科学研究所, 云南 景洪 666100
摘要:  为了解巴西橡胶树(Hevea brasiliensis)栽培种质的变异情况,以53份在云南植胶区综合性状表现较好的巴西橡胶树栽培种质为材料,采用流式细胞术测定了基因组C值,并进行了变异分析。结果表明,浅绿色嫩叶是巴西橡胶树流式细胞术测定的最适样品。53份巴西橡胶树栽培种质的细胞核DNA含量和基因组C值存在一定差异,基因组的平均C值是1.531 696×109bp,最小的是CRTG-272种质(1.465 908×109bp),最大的是CRTG-83种质(1.600 381×109bp),变异系数较小(CV=0.035 5)。53份巴西橡胶树栽培种质中有47份为二倍体,6份为三倍体。在已测定基因组大小的40种大戟科(Euphorbiaceae)植物中,基因组大小变异较大(CV=1.248 6),与“C值悖论”观点相一致。因此,应用流式细胞术能快速、准确地测定巴西橡胶树细胞核DNA含量、基因组C值和染色体倍性。
关键词:  巴西橡胶树  栽培种质  C  变异
DOI:10.11926/jtsb.3857
分类号:
基金项目:云南省现代农业天然橡胶产业技术体系建设项目;云南省技术创新人才培养对象项目;云南省热带作物科技创新体系建设项目(RF2018/RF2017)资助
Genome C Value and Variation Analysis of Cultivated Rubber Tree (Hevea brasiliensis) Germplasms by Flow Cytometry
LIU Jin, NIU Ying-feng, WU Yu, MAO Chang-li, ZHANG Feng-liang, LIU Zi-yan, ZHENG Cheng, LONG Qing-yi, LI Guo-hua
Yunnan Institute of Tropical Crops, Jinghong 666100, Yunnan, China
Abstract:  In order to understand the variation of cultivated germplasm of Hevea brasiliensis, the genomic C value of 53 cultivated germplasms with high quality characters in Yunnan rubber planting areas were measured by flow cytometry, and their variations were analyzed. The results showed that light green young leaves of H. brasiliensis were suitable samples for flow cytometry. There were differences in nuclear DNA content and genomic C value among 53 cultivated germplasms. The average genomic C value was 1.531 696×109bp, the smallest was CRTG-272 (1.465 908×109bp), the largest was CRTG-83 (1.600 381×109bp), and the variation coefficient (CV) was small (0.035 5). Among 53 cultivated germplasms of H. brasiliensis, forty-seven were diploid and 6 were triploid. The genome sizes had big CV up to 1.248 6 among 40 species of Euphorbiaceae, which is consistent with the view of the C value paradox. Therefore, flow cytometry can achieve rapid and accurate determination of the nuclear DNA content, genomic C value, and chromosome ploidy of H. brasiliensis.
Key words:  Hevea brasiliensis  Cultivated germplasm  C value  Variation

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