| 摘要: 为了解朱砂莲（Aristolochia tuberosa O.F.Liang et S.M.Huang）的化学成分，从其果实的甲醇提取物中分离得到4个化合物。通过波普数据分析，分别鉴定为马兜铃内酰胺W（1）、8-epidiosbulbin e acetate（2）、diosbulbin B（3）和β-sitosterol（4）。化合物1~3对南方根结线虫2龄幼虫具有不同程度的毒杀作用，尤其马兜铃内酰胺W（1）活性最好，其96 h后的LC50为119.94 μg mL-1。马兜铃属植物具有开发为新型植物源杀根结线虫剂的潜在价值。 |
| 关键词: 朱砂莲 果实 化学成分 南方根结线虫 杀根结线虫活性 |
| DOI：10.11926/jtsb.3842 |
| 分类号: |
| 基金项目:国家自然科学基金项目（31360447）；海南省重点研发计划项目（ZDYF2016157）资助 |
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| Nematicidal Activity of Chemical Compositions from Aristolochia tuberosa Fruits against Root-knot Nematode |
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BU Miao-miao, HAN De-jian, DONG Cun-zhu
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Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China
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Abstract: In order to understand the bioactivity constituents from Aristolochia tuberosa O. F. Liang et S. M. Huang, four compounds were isolated from methanol extract of A. tuberosa fruits by using silica gel column chromatography, Sephadex LH-20 gel column chromatography, precipitation and recrystallization methods. On the basis of spectral data, their structures were identified as aristololactam W (1), 8-epidiosbulbin e acetate (2), diosbulbin B (3) and β-sitosterol (4). Furthermore, nematicidal activity of four compounds against the second stage juvenile (J2) of Meloidogyne javanica were analyzed. The compounds 1-3 exhibited obviously toxic effects on J2 of M. javanica in vitro, especially for aristololactam W with the LC50 values of 119.94 μg mL-1 after 96 h. So, Aristolochia species have the potential value of developing new plant source to control root nematodes. |
| Key words: Aristolochia tuberosa Fruit Phytochemistry South root knot nematodes Nematicidal |
