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毛竹油菜素内酯受体激酶基因的分子特征及表达模式分析
王思宁, 孙化雨, 徐浩, 杨意宏, 赵韩生, 高志民
国家林业局竹藤科学与技术重点开放实验室, 国际竹藤中心竹藤资源基因科学研究所, 北京 100102
摘要:  为探究毛竹(Phyllostachys edulis)油菜素内酯(brassinolide,BL)受体激酶基因的分子特征和表达模式,采用生物信息学方法对毛竹中BL受体激酶基因进行了分析,并应用实时定量PCR技术对基因的表达模式进行了研究。结果表明,在毛竹基因组中共获得8条BL受体激酶基因同源序列(PeBRLs),分别属于4个亚家族。8个PeBRLs编码858~1 224氨基酸,分子量为92~130 kDa。PeBRLs结构相对保守,激酶区均具有BL受体激酶特有的3个保守结构域;除PeBRL1-1具有2个跨膜结构域外,其余PeBRLs只有1个跨膜结构域。8个PeBRLs全部定位在细胞膜上,属于典型的膜嵌合蛋白。实时定量PCR结果显示,每个亚家族成员基因的组织特异性表达模式基本一致,但不同亚家族之间差异明显;在不同发育阶段的竹笋中,PeBRLs的表达呈现为4种变化趋势。因此,8个PeBRLs在毛竹不同组织和笋的不同发育阶段可能发挥着不同的作用。
关键词:  毛竹  油菜素内酯受体激酶基因  生物信息学  实时定量PCR  组织特异性表达
DOI:10.11926/jtsb.3818
分类号:
基金项目:林业公益性行业科研专项经费项目(201504106);"十二五"农村领域国家科技计划项目(2015BAD04B01)资助
Molecular Characteristics and Expression Analysis of Brassinolide Receptor Kinase Genes in Phyllostachys edulis
WANG Si-ning, SUN Hua-yu, XU Hao, YANG Yi-hong, ZHAO Han-sheng, GAO Zhi-min
State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Center for Bamboo and Rattan, Beijing 100102, China
Abstract:  In order to understand the molecular characteristics and expression patterns of brassinolide (BL) receptor kinase genes in Phyllostachys edulis, the characters of BL receptor kinase genes in P. edulis were analyzed by using bioinformatics methods, and their expression patterns were studied by quantitative real-time PCR (qRT-PCR). The results showed that there were eight BL receptor kinase homologous genes named PeBRLs in P. edulis, belonging to four subfamilies. The PeBRLs encoded proteins contained 858-1 224 amino acid residues with molecular weight of 92-130 kDa. All PeBRLs contained three conserved domains in the kinase region endemic to BL receptor kinase, which indicating the structure of PeBRLs were relatively conservative. Moreover, they all had one transmembrane structure except PeBRL1-1 with two ones. All eight PeBRLs were predicted to be localized on the cell membrane belonging to the typical membrane chimeric protein. The results of qRT-PCR demonstrated that PeBRLs within subfamily had basically consistent expression patterns in different tissues, but there were obvious differences among PeBRLs in different subfamilies. The expression of PeBRLs showed four variation trends in bamboo shoots at different development stages. Therefore, it was suggested that PeBRLs might play different roles in different bamboo tissues and bamboo shoots at different development stages.
Key words:  Phyllostachys edulis  Brassinolide receptor kinase gene  Bioinformatics  Quantitative real-time PCR  Tissue specific expression

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