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植物生长调节剂对黑木相思优树腋芽增殖及生根的影响
黄烈健,王鸿,胡峰,施琼
中国林业科学研究院热带林业研究所,中国林业科学研究院热带林业研究所,广东省农业科学院作物研究所,中国林业科学研究院热带林业研究所
摘要:  为建立黑木相思(Acacia melanoxylon)快繁技术体系,以含1个腋芽的无菌茎段为材料,研究了植物生长调节剂对其增殖和生根的影响。结果表明,6-BA 极易诱导黑木相思愈伤组织形成,但芽长势较差,不利于腋芽增殖体系的建立。而生长素既能诱导黑木相思生根,又能诱导腋芽增殖;将无菌茎段接入MS+IAA 0.5 mg L-1+IBA 0.5 mg L-1培养基中培养20 d的生根率为98.41%,培养40 d的腋芽增殖倍数为2.36,单株繁殖系数为6.57。这是首次成功建立高效、简便的生根和增殖同步发生的黑木相思直接器官发生途径的组培技术体系。
关键词:  黑木相思  腋芽增殖  生根  直接器官发生  间接器官发生
DOI:10.11926/j.issn.1005-3395.2015.06.011
分类号:
基金项目:国家 "十二五"科技支撑计划项目(2012BAD01B0402)资助
Effects of Plant Growth Regulators on Axillary Bud Proliferation and Rooting of Acacia melanoxylon Elite Tree
Huang Liejian,Wang Hong,Hu Feng and Shi Qiong
Research Institute of Tropical Forestry,CAF,Research Institute of Tropical Forestry,CAF,Crop Research Institute,Guangdong Academy of Sciences,Research Institute of Tropical Forestry,CAF
Abstract:  In order to establish rapid propagation system of Acacia melanoxylon, the effects of plant growth regulators on its axillary bud multiplication and rooting were studied by using sterile stem segments with one axillary bud as explants. The results showed that the calli of A. melanoxylon were easily induced and bud growth was poor by 6-BA, indicating the disadvantage of axillary bud proliferation. The proliferation and rooting of Acacia melanoxylon axillary buds could be induced by auxin. The stem sections were cultured on MS+IAA 0.5 mg L-1+IBA 0.5 mg L-1 medium, rooting rate was 98.41% cultured for 20 d, and cultured for 40 d, the axillary bud multiplication and propagation coefficient were 2.36 and 6.57 per plantlet, respectively. It was successfully established the direct organogenesis technology system of A. melanoxylon for the first time with the advantages of high efficient, easy rooting and proliferation occur synchronous.
Key words:  Acacia melanoxylon  Axillary bud proliferation  Rooting  Direct organogenesis  Indirect organogenesis

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