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巴西橡胶树HbSUT3HbSUT5 mRNA在嫩茎和中脉中的原位杂交分析
周斌辉,秦云霞,唐朝荣,周率
海南大学农学院;中国热带农业科学院橡胶研究所,中国热带农业科学院橡胶研究所,中国热带农业科学院橡胶研究所,海南大学农学院
摘要:  为研究巴西橡胶树(Hevea brasiliensis)中HbSUT3HbSUT5基因的功能,采用地高辛标记的RNA探针与橡胶树嫩茎和中脉两种组织切片分别进行RNA原位杂交,对这2种SUT基因在组织中的表达区域与表达特点进行了分析。结果表明,在橡胶树嫩茎中,两个SUT基因主要在树皮的韧皮部和皮层细胞中表达;在中脉中,两个SUT基因在除木质部导管系统外的其它部位均有表达;HbSUT3基因在嫩茎和中脉中的表达量相近,而HbSUT5基因在嫩茎中的表达量远高于中脉。这些揭示HbSUT3HbSUT5基因可能广泛参与韧皮部装载、蔗糖运输与库细胞供给等活动,同时两个SUT基因也存在功能分化。
关键词:  巴西橡胶树  HbSUT3  HbSUT5  RNA原位杂交  表达模式
DOI:10.3969/j.issn.1005-3395.2013.03.010
分类号:
基金项目:国家自然科学基金项目(30760114); 中国热带农业科学院橡胶研究所基本科研业务费项目(1630022011002); 国家天然橡胶产业技术体系育种项目(CRAS-34)资助
In situ Hybridization Analysis of HbSUT3 and HbSUT5 mRNAs in Young Stems and Midribs of Hevea brasiliensis
zhoubinhui,qinyunxia,tangchaorong and zhoulv
College of Agronomy, Hainan University;Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences,Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences,Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences,College of Agronomy, Hainan University
Abstract:  In para rubber tree (Hevea brasiliensis), HbSUT3 plays the role of key sucrose transporter (SUT) in sucrose loading into laticifers and rubber productivity, whereas HbSUT5 is putatively involved in the regulation of sucrose loading into laticifers. To further investigate the functions of the two SUT genes, digoxigenin-labeled cRNA probes were prepared, and used by in situ hybridization to detect the distribution patterns of HbSUT3 and HbSUT5 mRNAs in tissues of rubber young stems and midribs. The results showed that the two SUT genes were mainly expressed in phloem and cortex of barks in young stems, whereas they were expressed in all tissues of midribs except for xylem. The expressions of HbSUT3 were similar between young stems and midribs, while the expression of HbSUT5 in young stems was much higher than that in midribs. It was suggested that HbSUT3 and HbSUT5 could involve in activities, such as phloem loading, sucrose transportation and supply to sink cells, and they also had functional differentiation.
Key words:  Hevea brasiliensis  HbSUT3  HbSUT5  RNA in situ hybridization  Expression pattern

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