首页 | 本刊简介 | 编委会 | 投稿征稿 | 期刊订阅 | 下载中心 | English
 
   
  论文快速检索

请输入关键词

 

 

   
  信息公告
海外撷英栏目开通,欢迎投稿

热带亚热带植物学报网络开通

访问总次数
14377922
今日访问
90
当前在线
0人
 
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 3402次   下载 1435 本文二维码信息
码上扫一扫!
分享到: 微信 更多
AhNCED1基因转化花生研究
覃铭,胡博,刘璨,李玲,罗虹
1. 华南师范大学生命科学学院,广东省植物发育生物工程重点实验室;2.北京师范大学珠海分校工程技术学院;3.华南师范大学生命科学学院,广东省植物发育生物工程重点实验室
摘要:  构建转化AhNCED1基因花生(Arachis hypogaea L.)过表达载体35S∷AhNCED1∷GUS,用OD600=0.8的LBA4404农杆菌液浸染汕油523,抗性芽诱导率达100%,PCR检测89株筛选苗,43株呈阳性,GUS检测阳性率为50%。转基因植株地上部分ABA含量增加;PEG胁迫10 h,转基因植株叶片AhNCED1蛋白表达增强,內源ABA水平积累,超氧化物水平降低。
关键词:  AhNCED1基因  超表达  转化  花生  ABA
DOI:10.3969/j.issn.1005-3395.2010.3.2444
分类号:
基金项目:广东省科技计划项目(20065030014); 广东省自然科学基金(06025049); 广东省教育厅自然科学重点项目(C10033);国家自然科学基金面上项目(30771297)
Transformation AhNCED1 Gene in Peanut (Arachis hypogaea L.)
Qin Ming1,2, HU Bo3, LIU Can4, LI Ling4, LUO Hong4
1.College of Life Sciences, South China Normal University, Guangdong Provincial Key Lab of Biotechnology for Plant Development;2.Engineering Technology School, Beijing Normal University Zhuhai;3.College of Life Sciences, South China Normal University, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development;4.College of Life Sciences, South China Normal University, Guangdong Provincial Key Lab of Biotechnology for Plant Development
Abstract:  9-cis-epoxycarotenoid dioxygenase (NCED) is the key enzyme of ABA biosynthesis in higher plant. The expression vector 35S∷AhNCED1∷GUS for peanut (Arachis hypogaea L.) was constructed. Then AhNCED1 gene was transformed into cotyledon of ‘Shanyou 523’, which is sensitive to drought stress, by immersing into Agrobacterium tumefciens strain LBA4404 bacterial suspension solution. The induction rate of adventitious buds with kanamycin-resistant was 100%, and in which there were 48.3% and 50% positive plantlets by PCR amplification and GUS histochemical identification, respectively. The ABA contents in aboveground organs of transgenic plants were higher than that in the wild (non-transformation) plants. When the leaves of the transgenic AhNCED1 plants subjected to PEG stress for 10 h, the expressions of AhNCED1 protein and endogenous ABA content were increased, as well as superoxide level decreased.
Key words:  AhNCED1 gene  Expression  Genetic transformation  Peanut  Abscisic acid

Copyright 2007 版权所有 中国科学院华南植物园《热带亚热带植物学报》编辑部
地址: 广州市天河区兴科路723号 中国科学院华南植物园 《热带亚热带植物学报》编辑部 邮编:510650 电话: 86 (0)20-3725 2514  传真:86 (0)20-3725 2642 E-mail: jtsb@scib.ac.cn
本系统由北京勤云科技发展有限公司设计
京ICP备09084417号-2