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龙眼花芽与叶芽差异蛋白分析
庞国彩,谢东丽,陈清西,陈伟,龙强
1.福建农林大学作物遗传育种与综合利用教育部重点实验室;2.生命科学学院;3.园艺学院
摘要:  以龙眼(Dimocarpus longan Lour.)花芽和叶芽为材料,对龙眼花芽与叶芽分化发育过程中的差异蛋白质进行分析和鉴定,并探讨其生物学功能。本研究共鉴定出10个差异蛋白质:ATP synthase beta subunit,fructokinase,LHCII type I chlorophyll a/b binding protein,peroxidase, ascorbate peroxidase,14-3-3 protein,14-3-3 family protein,putative cytosolic cysteine synthase 7,retrotransposon protein,putative,Ty1-copia subclass,Protein Group similar to late embryogenesis abundant proteins。这些差异蛋白质可能与龙眼花芽与叶芽的物质和能量代谢、自由基清除和抗氧化作用、信号转导和基础代谢、氨基酸代谢等生理过程密切相关。
关键词:  龙眼;花芽  叶芽;蛋白质组;双向电泳;质谱
DOI:10.3969/j.issn.1005-3395.2009.3.2191
分类号:
基金项目:国家自然科学基金资助项目(30571293);教育部博士点基金项目(200803890009);福建省自然科学基金项目(B0710006); 福建省教育厅科技计划项目(JA05236)
Analysis of Differential Proteins during Development Stages of Flower Bud and Leaf Bud in Longan
PANG Guo-cai1,2, XIE Dong-li1,2, CHEN Qing-xi3,4, CHEN Wei1,2, LONG Qiang1,2
1.Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops;2.College of Life Science, Fujian Agriculture and Forestry University;3.a. Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops;4.College of Horticulture, Fujian Agriculture and Forestry University
Abstract:  The proteomes during development stages of flower bud and leaf bud in longan (Dimocarpus longan Lour.) were isolated and identified by using 2-DE technology and MS analysis. Ten proteins were identified by MALDI-TOF-TOF/MS, such as ATP synthase beta subunit, fructokinase, LHCII type I chlorophyll a/b binding protein, peroxidase, ascorbate peroxidase, 14-3-3 protein, 14-3-3 family protein, putative cytosolic cysteine synthase 7, retrotransposon protein, putative, Ty1-copia subclass, protein group similar to late embryogenesis abundant proteins. These proteins might involve in substance and energy metabolism, scavenging and anti-oxidation of free radical, signal transduction and basic metabolism, amino acid metabolism and other physiological processes.
Key words:  Longan  Flower bud  Leaf bud  Proteome  Two-dimensional electrophoresis  Mass spectrometry

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