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米氏凯伦藻溶血毒素的溶血反应特征
崔伟民1, 杨维东1,2, 刘洁生1,2, 李宏业1
1.暨南大学生物工程学系;2.水体富营养化与赤潮防治广东省高等学校重点实验室
摘要:  探讨了温度、pH值、二价阳离子等对米氏凯伦藻(Karenia mikimotoi Hasen)溶血毒素溶血活性的影响,分析了米氏凯伦藻溶血毒素的溶血反应特征。结果表明,实验室培养米氏凯伦藻的溶血活性约为64.69±6.43 HU L-1,单个藻细胞的溶血活性为6.17±0.61×10-6 HU;在实验温度(0~37℃)下,溶血活性随温度的增加而增加;pH 6.0时的溶血活性最高;Cu2+、Mg2+、Mn2+、Ca2+、Co2+、Zn2+和Hg2+等对米氏凯伦藻的溶血活性的影响不同。离子浓度为5 mmol/L时,Hg2+的抑制作用最强。高浓度Hg2+对红细胞的集合效应不但阻止了Hg2+进入血细胞诱导的溶血作用,而且阻止了毒素对细胞膜的破坏,但这种抑制作用可被EDTA消除。
关键词:  米氏凯伦藻  溶血毒素  二价阳离子  pH  温度
DOI:10.3969/j.issn.1005-3395.2009.3.2187
分类号:
基金项目:国家自然科学基金-广东省联合基金重点项目(U0733006);国家自然科学基金项目(30470321)
Hemolytic Properties of Hemolytic Extracts from Karenia mikimotoi Hasen
CUI Wei-min1, Yang Wei Dong1,2, LIU Jie-sheng1,2, LI Hong-ye1
1.Department of Biotechnology, Jinan University;2.Guangdong Province’s Higher Education Key Laboratory of Eutrophication and Red Tid Control
Abstract:   The effects of temperature, pH and different divalent cations on the hemolysis induced by Karenia mikimotoi Hasen were studied, and the hemolytic properties were analyzed. The results showed that the hemolytic activity of K. mikimotoi was 64.69±6.43 HU L-1 and 6.17±0.61×10-6 HU cell-1. The hemolytic activity increased with the temperature enhanced at 0°C~37°C. pH had an obvious effect on the haemolytic activity, and there was the highest activity at pH 6.0. When the concentration was 5 mmol/L, the divalent cations such as Cu2+, Mg2+, Mn2+, Ca2+, Co2+, Zn2+ and Hg2+ had different inhibition on the hemolysis, which Hg2+ was the strongest inhibitor. High concentration of Hg2+ induced a rapid and cooperative cell aggregation, and then prevent penetration of Hg2+ into the cells, which did not only avoid any haemolysis induced by the Hg2+ entrance, but also inhibited the destruction by the hemolytic toxin. However, this phenomenon could be eliminated by EDTA.
Key words:  Karenia mikimotoi Hasen  Hemolytic toxin  Divalent cations  pH  Temperature

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