首页 | 本刊简介 | 编委会 | 投稿征稿 | 期刊订阅 | 下载中心 | English
 
   
  论文快速检索

请输入关键词

 

 

   
  信息公告
海外撷英栏目开通,欢迎投稿

热带亚热带植物学报网络开通

访问总次数
14377903
今日访问
80
当前在线
0人
 
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 2666次   下载 1651 本文二维码信息
码上扫一扫!
分享到: 微信 更多
非洲菊试管苗叶柄愈伤组织的诱导与分化研究
周俊1,2, 张 美1, 曾宋君1, 吴坤林1, 陈之林1
1.中国科学院华南植物园;2.中国科学院研究生院
摘要:  以非洲菊(Gerbera janesonii Bolus)品种‘Sunanda’试管苗幼叶的带叶叶柄为材料,研究了培养基、外植体类型和继代次数等因素对愈伤组织诱导及分化的影响。结果表明:培养基上附加不同植物生长调节剂所诱导出的愈伤组织在形态和分化能力上存在显著差异,叶柄基部诱导愈伤组织的最适培养基是MS+TDZ 0.3 mg L-1+NAA 0.1 mg L-1。外植体以叶片长度>0.5 cm, 叶片已舒展,颜色嫩绿的幼叶最佳,继代培养基以MS+ KT 1.0 mg L-1较适宜,愈伤组织在分化培养基MS+6-BA 2.0 mg L-1+NAA 0.1 mg L-1上分化出芽的同时还会增殖,分化率达87.4%,继代培养2次的愈伤组织分化率可提高至95%。不定芽在生根培养基1/2MS+IBA 0.6 mg L-1上的生根率达100%,试管苗移栽45 d后,成活率达97%以上。
关键词:  非洲菊  切花  组织培养  外植体,培养基
DOI:10.3969/j.issn.1005-3395.2009.3.2116
分类号:
基金项目:广州市科技计划项目(2006J1-C01312)
Studies on Regeneration System from Petiole of Gerbera jamesonii Bolus ‘Sunanda’ in vitro
ZHOU Jun1,2, ZHAGN Mei1, ZENG Song-jun1, WU Kun-lin1, CHEN Zhi-lin1
1.South China Botanical Garden, Chinese Academy of Sciences;2.Graduate University of Chinese Academy of Sciences
Abstract:  The rapid propagation system has been established for Gerbera jamesonii Bolus ‘Sunanda’ using leaves with petiole as explants. The results showed that the medium with different plant growth regulator had significantly different effects on callus morphology and differentiation capability. The optimum medium for inducing callus from the base of petioles was Murashige and Skoog (MS) medium containing TDZ 0.3 mg L-1 and NAA 0.1 mg L-1. The optimum explants was unfolded, light green leaves with length>0.5 cm. The subculture medium was MS supplemented with KT 1.0 mg L-1, and the induction rate was 65.9%. The adventitious bud could differentiate cultured on MS supplemented with 6-BA2.0 mg L-1 and NAA 0.1 mg L-1, and the differentiation rate was 87.4% in the first differentiation culture, and above 95% in the second. The half-strength MS medium supplemented with IBA 0.6 mg L-1 was optimum for inducing root and the rooting was up to 100 %. When plantlets with well-developed root system were transferred for 45 days, survival was up to 97%.
Key words:  Gerbera  Cut flower  Tissue culture  Explant  Medium

Copyright 2007 版权所有 中国科学院华南植物园《热带亚热带植物学报》编辑部
地址: 广州市天河区兴科路723号 中国科学院华南植物园 《热带亚热带植物学报》编辑部 邮编:510650 电话: 86 (0)20-3725 2514  传真:86 (0)20-3725 2642 E-mail: jtsb@scib.ac.cn
本系统由北京勤云科技发展有限公司设计
京ICP备09084417号-2