首页 | 本刊简介 | 编委会 | 投稿征稿 | 期刊订阅 | 下载中心 | English
 
   
  论文快速检索

请输入关键词

 

 

   
  信息公告
海外撷英栏目开通,欢迎投稿

热带亚热带植物学报网络开通

访问总次数
14697140
今日访问
327
当前在线
0人
 
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 2845次   下载 1347 本文二维码信息
码上扫一扫!
分享到: 微信 更多
珍稀药用和观赏植物地涌金莲的组织培养和快速繁殖(英文)
曾宋君,吴坤林,陈之林,段俊
作者单位
曾宋君,吴坤林,陈之林,段俊*  
摘要:  以地涌金莲吸芽为外植体建立了有效的快繁体系。外植体经灭菌处理后在MS+6-BA 4.0 mg L-1+NAA 0.2 mg L-1+维生素C 150 mg L-1+10%椰子乳+3%蔗糖培养基上能进行不定芽的诱导和增殖,培养60 d后每个芽平均能产生4.10个不定芽。第6代增殖后,丛生芽的增殖系数可达4.23。生根培养基以1/2MS+NAA 1.0 mg L-1+AC 50 mg L-1的效果较好。以沙:泥炭土:沙=1:1:1为基质移栽试管苗,成活率达到93.5%以上。通过12个月的组织培养已生产1 0000多株试管苗。
关键词:  地涌金莲  组织培养  微繁殖
DOI:10.3969/j.issn.1005-3395.2007.1.010
分类号:
基金项目:
Tissue Culture and Rapid Propagation of Musella lasiocarpa, a Rare Chinese Medicinal and Ornamental Herb
ZENG Song-jun, WU Kun-lin, CHEN Zhi-lin, DUAN Jun
Abstract:  An efficient and rapid micropropagation system by using auxiliary buds as explants has been established for Musella lasiocarpa. Murashige and Skoog (MS) medium supplemented with 4.0 mg L-1 6-benzylaminopurine, 0.2 mg L-1 1-naphthaleneacetic acid, 150 mg L-1 vitamin C, 10% coconut milk and 3% sucrose was suitable for shoot induction and proliferation from divided buds. Each explant produced an average of 4.10 shoots after 60-day culture. After the sixth subculture, the shoot proliferation rate of plantlets was 4.23-fold. The half-strength MS medium supplemented with 1.0 mg L-1 indole-3-butyric acid and 150 mg L-1 activated charcoal was suitable for rooting. When micropropagated plantlets with well-developed root systems were transferred to planting bed containing a mixture of sand, sieved peat and perlite (1:1:1; v/v) in a greenhouse, 93.5% of the plantlets survived. About 10 000 plantlets were produced successfully for field transfer after 12 months of culture initiation. This production system is useful for ex situ conservation and large-scale multiplication of M. lasiocarpa.
Key words:  Musella lasiocarpa  Tissue culture  Micropropagation

Copyright 2007 版权所有 中国科学院华南植物园《热带亚热带植物学报》编辑部
地址: 广州市天河区兴科路723号 中国科学院华南植物园 《热带亚热带植物学报》编辑部 邮编:510650 电话: 86 (0)20-3725 2514  传真:86 (0)20-3725 2642 E-mail: jtsb@scib.ac.cn
本系统由北京勤云科技发展有限公司设计
京ICP备09084417号-2