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逆境诱导水稻谷胱甘肽合成酶基因的表达研究
麦维军, 曾纪晴, 张明永, 蔡昭艳
作者单位
麦维军, 曾纪晴, 张明永*, 蔡昭艳  
摘要:  用RACE方法获得了全长的水稻谷胱甘肽合成酶(GS)基因的cDNA,并命名为OsGS(GeneBank accession No.:AY453405)。该cDNA全长1892bp,编码一个由540个氨基酸组成的多肽,预测其氨基端(N端)含有一段定位叶绿体的信号肽。比较水稻基因组定位结果表明OsGS基因位于水稻12号染色体短臂上,转录区全长6321bp,由12个外显子和11个内含子组成。通过RT—PCR对OsGS在水稻正常生长条件和逆境条件下的表达进行了研究。结果表明,在正常生长条件下,OsGS在水稻幼苗的根和叶以及抽穗期水稻的根中表达:但不在抽穗期水稻的叶、茎和幼穗中表达,这显示OsGS在水稻中的表达具有发育和组织特异性。利用抽穗期水稻的叶片为材料,经高温、干旱和重金属逆境处理后,OsGS在抽穗期叶片中的转录被诱导表达;而在盐、低温、伤害逆境下则不被诱导。在轻度和中度干旱胁迫4h后OsGS基因可被诱导表达。外源ABA处理也能够提高OsGS的转录水平,这显示OsGS可能是依赖ABA信号途径的环境胁迫诱导基因。
关键词:  水稻  谷胱甘肽合成酶基因  基因表达  胁迫
DOI:10.3969/j.issn.1005-3395.2006.6.001
分类号:
基金项目:
Stress Inducible Expression Analysis of Glutathione Synthetase Gene in Rice (Oryza sativa)
MAI Wei-jun, ZENG Ji-qing, ZHANG Ming-yong, ChI Zhao-yan
Abstract:  A full-length cDNA of rice (Oryza sativa L.) glutathione synthetase (GS) gene was obtained by 5'- RACE (rapid amplification of 5 '-complementary DNA ends), and the gene was named as OsGS (GeneBank accession No.: AY453405). The 1 892 bp cDNA contained an open reading flame (ORF) encoding a polypeptide of 540 amino acid residues with a predicted signal peptide of chloroplast. The alignment of OsGS cDNA with the mapped genome sequences office indicated that OsGS is located in the short arm office chromosome 12, and the transcript region (6 321 bp) of this gene consists of 12 extrons and 11 introns. The transcriptions of OsGS in rice under normal and stress conditions were studied by RT-PCR method. The results showed that, under normal conditions, the transcriptions of Os GS could be detected in roots, leaves of one-week old seedlings and roots of heading rice, but not in leaves, stems and spikes of heading rice. This result indicated that the expression of Os GS conforms to a spatio-temporal pattern. Under stress conditions, the expression of OsGS in the leaves of heading rice demonstrated that the transcription of OsGS was strongly induced by heat shock (40℃), cadmium and drought; but not by salt, low temperature and wound. Further transcription analysis showed that OsGS expression was strongly induced by mild and medium drought stress for 4 hours. In addition, the exogenous ABA could induce the transcription of OsGS in the leaves of flowering rice, suggesting that the response of OsGS to drought might be mediated by ABA-dependent pathway.
Key words:  Oryza sativa  Glutatione synthetase gene  Expression  Stress

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