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摘要: 以细裂银叶菊叶片为材料,进行愈伤组织的诱导、分化培养及生根诱导培养。结果表明:叶片愈伤组织的诱导以MS+2,4-D 2 mg L-1+BA 1 mg L-1+NAA 0.1 mg L-1培养基较好;分化培养以MS+BA 0.5 mg L-1+NAA 0.1 mg L-1为好;生根诱导以1/2MS+NAA 0.01 mg L-1效果最好。 |
关键词: 细裂银叶菊 组织培养 植株再生 |
DOI:10.3969/j.issn.1005-3395.2004.5.016 |
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In vitro Propagation of Senecio cineraria cv. Silver Dust |
GONG Wei, HU Ting-xing, GONG Yuan-bo, YU Zheng, WANG Mi-li, GU Yun-jie
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Abstract: The leaves of Senecio cineraria cv. Silver Dust were used to study the callus induction, shoots induction and rooting. It was shown that MS medium supplemented with 2 mg L-1 2,4-D+1 mg L-1 BA+0.1 mg L-1 NAA was good for callus induction, while MS medium containing 0.5 mg L-1 BA+0.1 mg L-1 NAA, half-strength MS medium containing 0.01 mg L-1 NAA gave best results for shoot induction and for rooting, respectively. |
Key words: Senecio cineraria cv. Silver Dust Tissue culture Plant regeneration |