首页 | 本刊简介 | 编委会 | 投稿征稿 | 期刊订阅 | 下载中心 | English
 
   
  论文快速检索

请输入关键词

 

 

   
  信息公告
海外撷英栏目开通,欢迎投稿

热带亚热带植物学报网络开通

访问总次数
14697104
今日访问
307
当前在线
0人
 
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 1508次   下载 1128 本文二维码信息
码上扫一扫!
分享到: 微信 更多
球形棕囊藻(Phaeocystis globosa)叶绿体psaA基因片段的序列分析
杨泽民, 章群, 谢数涛, 韩博平, 吕颂辉, Hodgkiss
作者单位
杨泽民, 章群*, 谢数涛, 韩博平, 吕颂辉, Hodgkiss  
摘要:  根据GenBank中检索到的南极棕囊藻(Phaeocystisantarctica)psaA基因序列设计psaAL和psaAR引物,对球形棕囊藻(Phaeocystisglobosa)的psaA基因片段进行PCR扩增并测序,获得了629bp的DNA序列。应用ClustalX对球形棕囊藻P1、P2株系和南极棕囊藻的psaA基因片段序列进行比对,结果表明,球形棕囊藻psaA基因片段序列无插入/缺失,核苷酸差异率为3.34%。应用DNAstar分析软件推断球形棕囊藻和南极棕囊藻的psaA基因对应的氨基酸序列和RNA二级结构,发现它们的氨基酸序列差异不大,序列中209个氨基酸只有1个发生了变化,其氨基酸变异率为0.48%;除部分结构域比较相似外,RNA二级结构上体现一定程度的差异,这可能对棕囊藻的分子分类研究有参考价值。因所获得的psaA基因片段序列及氨基酸序列具有种的极端保守性,不适宜用作Phaeocystis属种间的分子分类研究。
关键词:  球形棕囊藻  南极棕囊藻  psaA基因  DNA序列  氨基酸序列  RNA二级结构  藻类
DOI:10.3969/j.issn.1005-3395.2004.5.008
分类号:
基金项目:
Sequence Analyses of Chloroplastic psaA Gene Fragment from Phaeocystis globosa
YANG Ze-min, ZHANG Qun, XIE Shu-tao, HAN Bo-ping, LüSong-hui, Hodgkiss
Abstract:  Based on the complete psaA gene sequence of Phaeocystis antarctica in GenBank, two primers psaAL and psaAR were designed to amplify psaA gene sequence fragment of Phaeocystis globosa. An unambiguous sequence of 629 bp was gained,which was aligned with the complete psaA gene of Phaeocystis antarctica in Clustal X. The corresponding nucleotide dissimilarity of the two sequences was only 3.34%, and no insertion/ deletion was found. The relative amino acid sequence and RNA secondary structure were concluded in DNAstar. The concluded amino acid sequence was also conservative,and there was only one distinct base among 209 amino acids,with the difference of 0.48% .However,the concluded RNA secondary structures were quite unlike,apart from a few conservative domains. Perhaps the secondary structure of RNA may benefit the study on taxonomy of Phaeocysitis.It is considered that the DNA sequences and amino acid sequences of psaA are conservative, which is unadvisable to use for molecular interspecific classification ofthe genus Phaeocysitis.
Key words:  Phaeocysitis globosa  Phaeocysitis antarctica  psaA  DNA sequence  Amino acid sequence  Secondary structure of RNA  Algae

Copyright 2007 版权所有 中国科学院华南植物园《热带亚热带植物学报》编辑部
地址: 广州市天河区兴科路723号 中国科学院华南植物园 《热带亚热带植物学报》编辑部 邮编:510650 电话: 86 (0)20-3725 2514  传真:86 (0)20-3725 2642 E-mail: jtsb@scib.ac.cn
本系统由北京勤云科技发展有限公司设计
京ICP备09084417号-2